Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity.
Identifieur interne : 000388 ( Main/Exploration ); précédent : 000387; suivant : 000389Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity.
Auteurs : H C Fuchs [Autriche] ; B. Bohle ; Y. Dall'Antonia ; C. Radauer ; K. Hoffmann-Sommergruber ; A. Mari ; O. Scheiner ; W. Keller ; H. BreitenederSource :
- Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology [ 0954-7894 ] ; 2006.
Descripteurs français
- KwdFr :
- Activation des lymphocytes (immunologie), Adulte (MeSH), Allergènes (immunologie), Allergènes (isolement et purification), Antigènes végétaux (immunologie), Antigènes végétaux (isolement et purification), Cellules cultivées (MeSH), Cytoplasme (immunologie), Dichroïsme circulaire (MeSH), Escherichia coli (métabolisme), Humains (MeSH), Hypersensibilité alimentaire (immunologie), Immunoglobuline E (immunologie), Lymphocytes B (immunologie), Lymphocytes T (immunologie), Prolifération cellulaire (MeSH), Protéines recombinantes (immunologie), Prunus (immunologie), Périplasme (immunologie), Réaction antigène-anticorps (MeSH), Test ELISA (méthodes).
- MESH :
- immunologie : Activation des lymphocytes, Allergènes, Antigènes végétaux, Cytoplasme, Hypersensibilité alimentaire, Immunoglobuline E, Lymphocytes B, Lymphocytes T, Protéines recombinantes, Prunus, Périplasme.
- isolement et purification : Allergènes, Antigènes végétaux.
- métabolisme : Escherichia coli.
- méthodes : Test ELISA.
- Adulte, Cellules cultivées, Dichroïsme circulaire, Humains, Prolifération cellulaire, Réaction antigène-anticorps.
English descriptors
- KwdEn :
- Adult (MeSH), Allergens (immunology), Allergens (isolation & purification), Antigen-Antibody Reactions (MeSH), Antigens, Plant (immunology), Antigens, Plant (isolation & purification), B-Lymphocytes (immunology), Cell Proliferation (MeSH), Cells, Cultured (MeSH), Circular Dichroism (MeSH), Cytoplasm (immunology), Enzyme-Linked Immunosorbent Assay (methods), Escherichia coli (metabolism), Food Hypersensitivity (immunology), Humans (MeSH), Immunoglobulin E (immunology), Lymphocyte Activation (immunology), Periplasm (immunology), Prunus (immunology), Recombinant Proteins (immunology), T-Lymphocytes (immunology).
- MESH :
- chemical , immunology : Allergens, Antigens, Plant, Immunoglobulin E, Recombinant Proteins.
- chemical , isolation & purification : Allergens, Antigens, Plant.
- immunology : B-Lymphocytes, Cytoplasm, Food Hypersensitivity, Lymphocyte Activation, Periplasm, Prunus, T-Lymphocytes.
- metabolism : Escherichia coli.
- methods : Enzyme-Linked Immunosorbent Assay.
- Adult, Antigen-Antibody Reactions, Cell Proliferation, Cells, Cultured, Circular Dichroism, Humans.
Abstract
BACKGROUND
Cherry allergy is often reported in the context of allergy to other fruits of the Rosaceae family and pollinosis to trees because of cross-reactive allergens. Allergic reactions to cherry are reported by 19-29% of birch pollen-allergic patients. Pru av 2, identified as a thaumatin-like protein (TLP) from sweet cherry, was recognized by the majority of cherry-allergic patients in immunoblotting.
OBJECTIVES
In order to investigate the structural characteristics and the immunoglobulin (Ig)E- and T cell reactivity of cherry-derived TLP, recombinant Pru av 2 was expressed in Escherichia coli and natural Pru av 2 was purified.
METHODS
Parallel-His and FLAG expression vectors were used for recombinant production of Pru av 2 in the cytoplasm and the periplasm of E. coli. Natural Pru av 2 was purified from fresh cherries and verified by N-terminal sequencing. Structural characterization was performed using circular dichroism (CD) measurements, and the biologic activity was measured in a glucanase assay. Using cherry-specific sera, the IgE-binding ability of recombinant and natural Pru av 2 was investigated in IgE-ELISA and the T cell reactivity was studied in proliferation assays. Results Natural Pru av 2 revealed thaumatin-like structural features and bound IgE of 50% of cherry-allergic patients. It was demonstrated to be enzymatically active. Recombinant Pru av 2 expressed in the cytoplasm of E. coli exhibited a slightly different folding compared with the natural protein. It was not recognized by IgE from cherry-allergic subjects, but retained the ability to stimulate T lymphocytes. Periplasmic recombinant Pru av 2 was able to bind an anti-grape TLP antibody and cherry-specific IgE.
CONCLUSIONS
We prepared two recombinant model TLPs from cherry, and compared their molecular characteristics as well as their IgE-binding activity and T cell interactions in relation to the natural counterpart. The cytoplasmic recombinant Pru av 2 can be used as a hypoallergenic variant in allergen-specific immunotherapy, whereas the periplasmic protein can be included in a component-resolved diagnosis.
DOI: 10.1111/j.1365-2222.2006.02439.x
PubMed: 16499648
Affiliations:
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Bohle</name></author><author><name sortKey="Dall Antonia, Y" sort="Dall Antonia, Y" uniqKey="Dall Antonia Y" first="Y" last="Dall'Antonia">Y. Dall'Antonia</name></author><author><name sortKey="Radauer, C" sort="Radauer, C" uniqKey="Radauer C" first="C" last="Radauer">C. Radauer</name></author><author><name sortKey="Hoffmann Sommergruber, K" sort="Hoffmann Sommergruber, K" uniqKey="Hoffmann Sommergruber K" first="K" last="Hoffmann-Sommergruber">K. Hoffmann-Sommergruber</name></author><author><name sortKey="Mari, A" sort="Mari, A" uniqKey="Mari A" first="A" last="Mari">A. Mari</name></author><author><name sortKey="Scheiner, O" sort="Scheiner, O" uniqKey="Scheiner O" first="O" last="Scheiner">O. Scheiner</name></author><author><name sortKey="Keller, W" sort="Keller, W" uniqKey="Keller W" first="W" last="Keller">W. Keller</name></author><author><name sortKey="Breiteneder, H" sort="Breiteneder, H" uniqKey="Breiteneder H" first="H" last="Breiteneder">H. Breiteneder</name></author></analytic><series><title level="j">Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology</title><idno type="ISSN">0954-7894</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult (MeSH)</term><term>Allergens (immunology)</term><term>Allergens (isolation & purification)</term><term>Antigen-Antibody Reactions (MeSH)</term><term>Antigens, Plant (immunology)</term><term>Antigens, Plant (isolation & purification)</term><term>B-Lymphocytes (immunology)</term><term>Cell Proliferation (MeSH)</term><term>Cells, Cultured (MeSH)</term><term>Circular Dichroism (MeSH)</term><term>Cytoplasm (immunology)</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Escherichia coli (metabolism)</term><term>Food Hypersensitivity (immunology)</term><term>Humans (MeSH)</term><term>Immunoglobulin E (immunology)</term><term>Lymphocyte Activation (immunology)</term><term>Periplasm (immunology)</term><term>Prunus (immunology)</term><term>Recombinant Proteins (immunology)</term><term>T-Lymphocytes (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Activation des lymphocytes (immunologie)</term><term>Adulte (MeSH)</term><term>Allergènes (immunologie)</term><term>Allergènes (isolement et purification)</term><term>Antigènes végétaux (immunologie)</term><term>Antigènes végétaux (isolement et purification)</term><term>Cellules cultivées (MeSH)</term><term>Cytoplasme (immunologie)</term><term>Dichroïsme circulaire (MeSH)</term><term>Escherichia coli (métabolisme)</term><term>Humains (MeSH)</term><term>Hypersensibilité alimentaire (immunologie)</term><term>Immunoglobuline E (immunologie)</term><term>Lymphocytes B (immunologie)</term><term>Lymphocytes T (immunologie)</term><term>Prolifération cellulaire (MeSH)</term><term>Protéines recombinantes (immunologie)</term><term>Prunus (immunologie)</term><term>Périplasme (immunologie)</term><term>Réaction antigène-anticorps (MeSH)</term><term>Test ELISA (méthodes)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Allergens</term><term>Antigens, Plant</term><term>Immunoglobulin E</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Allergens</term><term>Antigens, Plant</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Activation des lymphocytes</term><term>Allergènes</term><term>Antigènes végétaux</term><term>Cytoplasme</term><term>Hypersensibilité alimentaire</term><term>Immunoglobuline E</term><term>Lymphocytes B</term><term>Lymphocytes T</term><term>Protéines recombinantes</term><term>Prunus</term><term>Périplasme</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term><term>Cytoplasm</term><term>Food Hypersensitivity</term><term>Lymphocyte Activation</term><term>Periplasm</term><term>Prunus</term><term>T-Lymphocytes</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Allergènes</term><term>Antigènes végétaux</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Test ELISA</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Antigen-Antibody Reactions</term><term>Cell Proliferation</term><term>Cells, Cultured</term><term>Circular Dichroism</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Cellules cultivées</term><term>Dichroïsme circulaire</term><term>Humains</term><term>Prolifération cellulaire</term><term>Réaction antigène-anticorps</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b></p><p>Cherry allergy is often reported in the context of allergy to other fruits of the Rosaceae family and pollinosis to trees because of cross-reactive allergens. Allergic reactions to cherry are reported by 19-29% of birch pollen-allergic patients. Pru av 2, identified as a thaumatin-like protein (TLP) from sweet cherry, was recognized by the majority of cherry-allergic patients in immunoblotting.</p></div><div type="abstract" xml:lang="en"><p><b>OBJECTIVES</b></p><p>In order to investigate the structural characteristics and the immunoglobulin (Ig)E- and T cell reactivity of cherry-derived TLP, recombinant Pru av 2 was expressed in Escherichia coli and natural Pru av 2 was purified.</p></div><div type="abstract" xml:lang="en"><p><b>METHODS</b></p><p>Parallel-His and FLAG expression vectors were used for recombinant production of Pru av 2 in the cytoplasm and the periplasm of E. coli. Natural Pru av 2 was purified from fresh cherries and verified by N-terminal sequencing. Structural characterization was performed using circular dichroism (CD) measurements, and the biologic activity was measured in a glucanase assay. Using cherry-specific sera, the IgE-binding ability of recombinant and natural Pru av 2 was investigated in IgE-ELISA and the T cell reactivity was studied in proliferation assays. Results Natural Pru av 2 revealed thaumatin-like structural features and bound IgE of 50% of cherry-allergic patients. It was demonstrated to be enzymatically active. Recombinant Pru av 2 expressed in the cytoplasm of E. coli exhibited a slightly different folding compared with the natural protein. It was not recognized by IgE from cherry-allergic subjects, but retained the ability to stimulate T lymphocytes. Periplasmic recombinant Pru av 2 was able to bind an anti-grape TLP antibody and cherry-specific IgE.</p></div><div type="abstract" xml:lang="en"><p><b>CONCLUSIONS</b></p><p>We prepared two recombinant model TLPs from cherry, and compared their molecular characteristics as well as their IgE-binding activity and T cell interactions in relation to the natural counterpart. The cytoplasmic recombinant Pru av 2 can be used as a hypoallergenic variant in allergen-specific immunotherapy, whereas the periplasmic protein can be included in a component-resolved diagnosis.</p></div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16499648</PMID><DateCompleted><Year>2006</Year><Month>08</Month><Day>21</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0954-7894</ISSN><JournalIssue CitedMedium="Print"><Volume>36</Volume><Issue>3</Issue><PubDate><Year>2006</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology</Title><ISOAbbreviation>Clin Exp Allergy</ISOAbbreviation></Journal><ArticleTitle>Natural and recombinant molecules of the cherry allergen Pru av 2 show diverse structural and B cell characteristics but similar T cell reactivity.</ArticleTitle><Pagination><MedlinePgn>359-68</MedlinePgn></Pagination><Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Cherry allergy is often reported in the context of allergy to other fruits of the Rosaceae family and pollinosis to trees because of cross-reactive allergens. Allergic reactions to cherry are reported by 19-29% of birch pollen-allergic patients. Pru av 2, identified as a thaumatin-like protein (TLP) from sweet cherry, was recognized by the majority of cherry-allergic patients in immunoblotting.</AbstractText><AbstractText Label="OBJECTIVES" NlmCategory="OBJECTIVE">In order to investigate the structural characteristics and the immunoglobulin (Ig)E- and T cell reactivity of cherry-derived TLP, recombinant Pru av 2 was expressed in Escherichia coli and natural Pru av 2 was purified.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">Parallel-His and FLAG expression vectors were used for recombinant production of Pru av 2 in the cytoplasm and the periplasm of E. coli. Natural Pru av 2 was purified from fresh cherries and verified by N-terminal sequencing. Structural characterization was performed using circular dichroism (CD) measurements, and the biologic activity was measured in a glucanase assay. Using cherry-specific sera, the IgE-binding ability of recombinant and natural Pru av 2 was investigated in IgE-ELISA and the T cell reactivity was studied in proliferation assays. Results Natural Pru av 2 revealed thaumatin-like structural features and bound IgE of 50% of cherry-allergic patients. It was demonstrated to be enzymatically active. Recombinant Pru av 2 expressed in the cytoplasm of E. coli exhibited a slightly different folding compared with the natural protein. It was not recognized by IgE from cherry-allergic subjects, but retained the ability to stimulate T lymphocytes. Periplasmic recombinant Pru av 2 was able to bind an anti-grape TLP antibody and cherry-specific IgE.</AbstractText><AbstractText Label="CONCLUSIONS" NlmCategory="CONCLUSIONS">We prepared two recombinant model TLPs from cherry, and compared their molecular characteristics as well as their IgE-binding activity and T cell interactions in relation to the natural counterpart. The cytoplasmic recombinant Pru av 2 can be used as a hypoallergenic variant in allergen-specific immunotherapy, whereas the periplasmic protein can be included in a component-resolved diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fuchs</LastName><ForeName>H C</ForeName><Initials>HC</Initials><AffiliationInfo><Affiliation>Center of Physiology and Pathophysiology, Medical University of Vienna, Vienna, Austria.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bohle</LastName><ForeName>B</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Dall'Antonia</LastName><ForeName>Y</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Radauer</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Hoffmann-Sommergruber</LastName><ForeName>K</ForeName><Initials>K</Initials></Author><Author ValidYN="Y"><LastName>Mari</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Scheiner</LastName><ForeName>O</ForeName><Initials>O</Initials></Author><Author ValidYN="Y"><LastName>Keller</LastName><ForeName>W</ForeName><Initials>W</Initials></Author><Author ValidYN="Y"><LastName>Breiteneder</LastName><ForeName>H</ForeName><Initials>H</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Clin Exp Allergy</MedlineTA><NlmUniqueID>8906443</NlmUniqueID><ISSNLinking>0954-7894</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000485">Allergens</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D052179">Antigens, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C510168">Pru av 2 allergen, Prunus avium</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>37341-29-0</RegistryNumber><NameOfSubstance UI="D007073">Immunoglobulin E</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000328" MajorTopicYN="N">Adult</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000485" MajorTopicYN="N">Allergens</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000937" MajorTopicYN="N">Antigen-Antibody Reactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D052179" MajorTopicYN="N">Antigens, Plant</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001402" MajorTopicYN="N">B-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002478" MajorTopicYN="N">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002942" MajorTopicYN="N">Circular Dichroism</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003593" MajorTopicYN="N">Cytoplasm</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005512" MajorTopicYN="N">Food Hypersensitivity</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007073" MajorTopicYN="N">Immunoglobulin E</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008213" MajorTopicYN="N">Lymphocyte Activation</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019897" MajorTopicYN="N">Periplasm</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027861" MajorTopicYN="N">Prunus</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013601" MajorTopicYN="N">T-Lymphocytes</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>8</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>2</Month><Day>28</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16499648</ArticleId><ArticleId IdType="pii">CEA2439</ArticleId><ArticleId IdType="doi">10.1111/j.1365-2222.2006.02439.x</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Autriche</li></country><region><li>Vienne (Autriche)</li></region><settlement><li>Vienne (Autriche)</li></settlement></list><tree><noCountry><name sortKey="Bohle, B" sort="Bohle, B" uniqKey="Bohle B" first="B" last="Bohle">B. Bohle</name><name sortKey="Breiteneder, H" sort="Breiteneder, H" uniqKey="Breiteneder H" first="H" last="Breiteneder">H. Breiteneder</name><name sortKey="Dall Antonia, Y" sort="Dall Antonia, Y" uniqKey="Dall Antonia Y" first="Y" last="Dall'Antonia">Y. Dall'Antonia</name><name sortKey="Hoffmann Sommergruber, K" sort="Hoffmann Sommergruber, K" uniqKey="Hoffmann Sommergruber K" first="K" last="Hoffmann-Sommergruber">K. Hoffmann-Sommergruber</name><name sortKey="Keller, W" sort="Keller, W" uniqKey="Keller W" first="W" last="Keller">W. Keller</name><name sortKey="Mari, A" sort="Mari, A" uniqKey="Mari A" first="A" last="Mari">A. Mari</name><name sortKey="Radauer, C" sort="Radauer, C" uniqKey="Radauer C" first="C" last="Radauer">C. Radauer</name><name sortKey="Scheiner, O" sort="Scheiner, O" uniqKey="Scheiner O" first="O" last="Scheiner">O. Scheiner</name></noCountry><country name="Autriche"><region name="Vienne (Autriche)"><name sortKey="Fuchs, H C" sort="Fuchs, H C" uniqKey="Fuchs H" first="H C" last="Fuchs">H C Fuchs</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:16499648" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ThaumatinV1
| This area was generated with Dilib version V0.6.37. |  |